Blocking the binding of WT1 to bcl-2 promoter by G-quadruplex ligand SYUIQ-FM05

نویسندگان

  • Yun-Xia Xiong
  • Ai-Chun Chen
  • Pei-Fen Yao
  • De-Ying Zeng
  • Yu-Jing Lu
  • Jia-Heng Tan
  • Zhi-Shu Huang
  • Tian-Miao Ou
چکیده

At present, wt1, a Wilms' tumor suppressor gene, is recognized as a critical regulator of tumorigenesis and a potential therapeutic target. WT1 shows the ability to regulate the transcription of bcl-2 by binding to a GC-rich region in the promoter, which can then fold into a special DNA secondary structure called the G-quadruplex. This function merits the exploration of the effect of a G-quadruplex ligand on the binding and subsequent regulation of WT1 on the bcl-2 promoter. In the present study, WT1 was found to bind to the double strand containing the G-quadruplex-forming sequence of the bcl-2 promoter. However, the G-quadruplex ligand SYUIQ-FM05 effectively blocked this binding by interacting with the GC-rich sequence. Our new findings are significant in the exploration of new strategies to block WT1's transcriptional regulation for cancer-cell treatment.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2016